diff --git a/packages/plugins/i18n/server/constants/__tests__/index.test.js b/packages/plugins/i18n/server/constants/__tests__/index.test.js
index e0618330c6..c94bdb58cc 100644
--- a/packages/plugins/i18n/server/constants/__tests__/index.test.js
+++ b/packages/plugins/i18n/server/constants/__tests__/index.test.js
@@ -1,6 +1,6 @@
 'use strict';
 
-const { getInitLocale } = require('..');
+const { getInitLocale, isoLocales } = require('..');
 
 describe('I18N default locale', () => {
   describe('getInitLocale', () => {
@@ -23,5 +23,10 @@ describe('I18N default locale', () => {
       process.env.STRAPI_PLUGIN_I18N_INIT_LOCALE_CODE = 'zzzzz';
       expect(() => getInitLocale()).toThrow();
     });
+
+    test('Checks if there are any duplicate locales present in the "iso-locales.json" file', () => {
+      const set = new Set(isoLocales.map((item) => item.code)).size !== isoLocales.length;
+      expect(set).toBe(false);
+    });
   });
 });
diff --git a/packages/plugins/i18n/server/constants/iso-locales.json b/packages/plugins/i18n/server/constants/iso-locales.json
index 67c15bbcc7..9ad9618daa 100644
--- a/packages/plugins/i18n/server/constants/iso-locales.json
+++ b/packages/plugins/i18n/server/constants/iso-locales.json
@@ -591,22 +591,6 @@
     "code":"en-GG",
     "name":"English (Guernsey) (en-GG)"
   },
-  {
-    "code":"en-CA",
-    "name":"English (Canada) (en-CA)"
-  },
-  {
-    "code":"en-EG",
-    "name":"English (Egypt) (en-EG)"
-  },
-  {
-    "code":"en-EU",
-    "name":"English (Europe) (en-EU)"
-  },
-  {
-    "code":"en-GU",
-    "name":"English (Guam) (en-GU)"
-  },
   {
     "code":"en-GY",
     "name":"English (Guyana) (en-GY)"
@@ -667,10 +651,6 @@
     "code":"en-MY",
     "name":"English (Malaysia) (en-MY)"
   },
-  {
-    "code":"en-JM",
-    "name":"English (Jamaica) (en-JM)"
-  },
   {
     "code":"en-MT",
     "name":"English (Malta) (en-MT)"
@@ -719,14 +699,6 @@
     "code":"en-NF",
     "name":"English (Norfolk Island) (en-NF)"
   },
-  {
-    "code":"en-NA",
-    "name":"English (Namibia) (en-NA)"
-  },
-  {
-    "code":"en-NZ",
-    "name":"English (New Zealand) (en-NZ)"
-  },
   {
     "code":"en-MP",
     "name":"English (Northern Mariana Islands) (en-MP)"
@@ -795,10 +767,6 @@
     "code":"en-SB",
     "name":"English (Solomon Islands) (en-SB)"
   },
-  {
-    "code":"en-ZA",
-    "name":"English (South Africa) (en-ZA)"
-  },
   {
     "code":"en-SS",
     "name":"English (South Sudan) (en-SS)"
@@ -859,26 +827,10 @@
     "code":"en-TV",
     "name":"English (Tuvalu) (en-TV)"
   },
-  {
-    "code":"en-PH",
-    "name":"English (Philippines) (en-PH)"
-  },
-  {
-    "code":"en-SA",
-    "name":"English (Saudi Arabia) (en-SA)"
-  },
-  {
-    "code":"en-SG",
-    "name":"English (Singapore) (en-SG)"
-  },
   {
     "code":"en-ZA",
     "name":"English (South Africa) (en-ZA)"
   },
-  {
-    "code":"en-TT",
-    "name":"English (Trinidad and Tobago) (en-TT)"
-  },
   {
     "code":"en-AE",
     "name":"English (U.A.E.) (en-AE)"
diff --git a/packages/plugins/i18n/server/services/__tests__/__snapshots__/iso-locales.test.js.snap b/packages/plugins/i18n/server/services/__tests__/__snapshots__/iso-locales.test.js.snap
index 7484dcef95..c5a1d2dfb7 100644
--- a/packages/plugins/i18n/server/services/__tests__/__snapshots__/iso-locales.test.js.snap
+++ b/packages/plugins/i18n/server/services/__tests__/__snapshots__/iso-locales.test.js.snap
@@ -594,22 +594,6 @@ exports[`ISO locales getIsoLocales 1`] = `
     "code": "en-GG",
     "name": "English (Guernsey) (en-GG)",
   },
-  {
-    "code": "en-CA",
-    "name": "English (Canada) (en-CA)",
-  },
-  {
-    "code": "en-EG",
-    "name": "English (Egypt) (en-EG)",
-  },
-  {
-    "code": "en-EU",
-    "name": "English (Europe) (en-EU)",
-  },
-  {
-    "code": "en-GU",
-    "name": "English (Guam) (en-GU)",
-  },
   {
     "code": "en-GY",
     "name": "English (Guyana) (en-GY)",
@@ -670,10 +654,6 @@ exports[`ISO locales getIsoLocales 1`] = `
     "code": "en-MY",
     "name": "English (Malaysia) (en-MY)",
   },
-  {
-    "code": "en-JM",
-    "name": "English (Jamaica) (en-JM)",
-  },
   {
     "code": "en-MT",
     "name": "English (Malta) (en-MT)",
@@ -722,14 +702,6 @@ exports[`ISO locales getIsoLocales 1`] = `
     "code": "en-NF",
     "name": "English (Norfolk Island) (en-NF)",
   },
-  {
-    "code": "en-NA",
-    "name": "English (Namibia) (en-NA)",
-  },
-  {
-    "code": "en-NZ",
-    "name": "English (New Zealand) (en-NZ)",
-  },
   {
     "code": "en-MP",
     "name": "English (Northern Mariana Islands) (en-MP)",
@@ -798,10 +770,6 @@ exports[`ISO locales getIsoLocales 1`] = `
     "code": "en-SB",
     "name": "English (Solomon Islands) (en-SB)",
   },
-  {
-    "code": "en-ZA",
-    "name": "English (South Africa) (en-ZA)",
-  },
   {
     "code": "en-SS",
     "name": "English (South Sudan) (en-SS)",
@@ -862,26 +830,10 @@ exports[`ISO locales getIsoLocales 1`] = `
     "code": "en-TV",
     "name": "English (Tuvalu) (en-TV)",
   },
-  {
-    "code": "en-PH",
-    "name": "English (Philippines) (en-PH)",
-  },
-  {
-    "code": "en-SA",
-    "name": "English (Saudi Arabia) (en-SA)",
-  },
-  {
-    "code": "en-SG",
-    "name": "English (Singapore) (en-SG)",
-  },
   {
     "code": "en-ZA",
     "name": "English (South Africa) (en-ZA)",
   },
-  {
-    "code": "en-TT",
-    "name": "English (Trinidad and Tobago) (en-TT)",
-  },
   {
     "code": "en-AE",
     "name": "English (U.A.E.) (en-AE)",